With the completion of the human genome project, single-nucleotide polymorphisms (SNPs) have become the focus of intense study in biomedical research. Polymerase-mediated primer extension has been ...

Mutations are a hallmark of cancer. Normal cells minimize spontaneous mutations through the combined actions of polymerase base selectivity, 3′ → 5′ exonucleolytic proofreading, mismatch correction, ...

Researchers from Tokyo Metropolitan University have identified key factors in the mechanism behind DNA repair in our bodies. For the first time, they showed that the "proofreading" portion of the DNA ...

When an SSB appears, polymerase epsilon approaches the nick before retracing its steps thanks to its exonuclease, allowing fork reversal to take place. The nick is repaired as an SSB, and replication ...

As expected, accuracy also depended on whether the DNA polymerase retains a 'proofreading' functionality, which verifies (and if necessary removes) any mismatched nucleotides: a version of the enzyme ...

One of the hallmarks of many DNA polymerases is that they proofread as they copy DNA, ensuring high-fidelity duplicates of an organism’s genome. This error-checking ability is missing from enzymes ...

Abbondanzieri) For a high resolution photograph click here. When Ralph Waldo Emerson said that nature pardons no mistakes, he wasn't thinking about RNA polymerase (RNAP) - the versatile enzyme that ...

Despite the efforts of a league of DNA proofreading and copyediting enzymes, the genetic code that keeps us all alive is not impervious to mutation. Some mutations are innocuous, while others can cut ...

Madison, Wis.-based Novagen now sells KOD HiFi DNA polymerase (manufactured by Toyobo of Japan), a proofreading enzyme for high-fidelity PCR amplification. KOD HiFi DNA polymerase is a recombinant ...

Thermo Fisher Scientific Inc. has announced the new Thermo Scientific Verbatim High Fidelity DNA Polymerase. According to Thermo Fisher Scientificm this advanced DNA polymerase allows highly accurate ...